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Western Blotting Analysis Proteins from the whole kidney lysates were separated on a 10% SDS-polyacrylamide gel, transferred onto PVDF membrane, blocked in 5% BSA for 60min, and then primary antibodies against KIM-1, IL-1, TNF-, Ferritin, FPN, xCT, and p53 at the dilution of 1:1,000, as well as 4-HNE, GPX4, and -actin at the dilution of 1:4,000 were probed at 4C overnight

To shorten the description, the use of NADPH at several concentrations is not described here, but was performed by the same statistical methods of analysis previously described for GP

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Other Ingredients: De-ionized Water, Vegetable Glycerin, Malic Acid, Citric Acid, Natural Flavors, Potassium Sorbate (as preservative) and Lemon (Citrus limon) Oil